E-ISSN 2218-6050 | ISSN 2226-4485
 
2024, Vol: 14, Issue: 11
14 / 11Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submission Form

Research Article


Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia

Safira Ramadhani, Aswin Rafif Khairullah, Mustofa Helmi Effendi, Mohammad Sukmanadi, Wiwiek Tyasningsih, Saumi Kirey Millannia, Daniah Ashri Afnani, Ikechukwu Benjamin Moses, M. Thoriq Ihza Farizqi, Sergius Erikson Kaben.


Abstract
Background:
The most susceptible group of people to spread methicillin-resistant Staphylococcus aureus (MRSA) among domestic cats is their owners' relatives.

Aim:
Considering the aforementioned, research at the Surabaya City Animal Hospital is necessary to determine whether the mecA gene may be detected in cat nasal swabs.

Methods:
Samples were taken using a sterile cotton swab, and the transport medium was buffered peptone water. Standard operating procedures in microbiology were then employed to identify the samples. Using five distinct antibiotic discs, the Kirby-Bauer diffusion method was used to determine the antibiotic resistance profile of S. aureus. All cefoxitin-resistant S. aureus isolates were tested for MRSA and then the mecA gene was detected.

Results:
Based on morphological culture features, Gram staining, and biochemical assays, 64 samples (64%) of the 100 isolated cat nose swab samples were positive for S. aureus, according to the results of the biochemical tests. The profile of antibiotic resistance developed from the results of the S. aureus resistance test to antibiotics revealed that five (7.81%) of the 64 S. aureus isolates were confirmed to be multidrug resistant. Out of the 6 MRSA isolates analyzed, the mecA gene was detected in 5 isolates.

Conclusion:
Based on the findings of this study, a thorough assessment of the impact of using antibiotics to prevent the spread of MRSA in veterinary hospitals on the general public's health is required in light of the detection of MRSA isolates bearing the mecA gene in the Surabaya veterinary hospital.

Key words: Antibiotic, Cat, MDR, MRSA, Public health


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Safira Ramadhani
Articles by Aswin Rafif Khairullah
Articles by Mustofa Helmi Effendi
Articles by Mohammad Sukmanadi
Articles by Wiwiek Tyasningsih
Articles by Saumi Kirey Millannia
Articles by Daniah Ashri Afnani
Articles by Ikechukwu Benjamin Moses
Articles by M. Thoriq Ihza Farizqi
Articles by Sergius Erikson Kaben
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Ramadhani S, Khairullah AR, Effendi MH, Sukmanadi M, Tyasningsih W, Millannia SK, Afnani DA, Moses IB, Farizqi MTI, Kaben SE. Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. Open Vet J. 2024; 14(11): 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31

Web Style

Ramadhani S, Khairullah AR, Effendi MH, Sukmanadi M, Tyasningsih W, Millannia SK, Afnani DA, Moses IB, Farizqi MTI, Kaben SE. Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. https://www.openveterinaryjournal.com/?mno=217834 [Access: December 06, 2024]. doi:10.5455/OVJ.2024.v14.i11.31

AMA (American Medical Association) Style

Ramadhani S, Khairullah AR, Effendi MH, Sukmanadi M, Tyasningsih W, Millannia SK, Afnani DA, Moses IB, Farizqi MTI, Kaben SE. Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. Open Vet J. 2024; 14(11): 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31


Vancouver/ICMJE Style

Ramadhani S, Khairullah AR, Effendi MH, Sukmanadi M, Tyasningsih W, Millannia SK, Afnani DA, Moses IB, Farizqi MTI, Kaben SE. Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. Open Vet J. (2024), [cited December 06, 2024]; 14(11): 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31


Harvard Style

Ramadhani, S., Khairullah, . A. R., Effendi, . M. H., Sukmanadi, . M., Tyasningsih, . W., Millannia, . S. K., Afnani, . D. A., Moses, . I. B., Farizqi, . M. T. I. & Kaben, . S. E. (2024) Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. Open Vet J, 14 (11), 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31


Turabian Style

Ramadhani, Safira, Aswin Rafif Khairullah, Mustofa Helmi Effendi, Mohammad Sukmanadi, Wiwiek Tyasningsih, Saumi Kirey Millannia, Daniah Ashri Afnani, Ikechukwu Benjamin Moses, M. Thoriq Ihza Farizqi, and Sergius Erikson Kaben. 2024. Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. Open Veterinary Journal, 14 (11), 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31


Chicago Style

Ramadhani, Safira, Aswin Rafif Khairullah, Mustofa Helmi Effendi, Mohammad Sukmanadi, Wiwiek Tyasningsih, Saumi Kirey Millannia, Daniah Ashri Afnani, Ikechukwu Benjamin Moses, M. Thoriq Ihza Farizqi, and Sergius Erikson Kaben. "Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia." Open Veterinary Journal 14 (2024), 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31


MLA (The Modern Language Association) Style

Ramadhani, Safira, Aswin Rafif Khairullah, Mustofa Helmi Effendi, Mohammad Sukmanadi, Wiwiek Tyasningsih, Saumi Kirey Millannia, Daniah Ashri Afnani, Ikechukwu Benjamin Moses, M. Thoriq Ihza Farizqi, and Sergius Erikson Kaben. "Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia." Open Veterinary Journal 14.11 (2024), 3026-3036. Print. doi:10.5455/OVJ.2024.v14.i11.31


APA (American Psychological Association) Style

Ramadhani, S., Khairullah, . A. R., Effendi, . M. H., Sukmanadi, . M., Tyasningsih, . W., Millannia, . S. K., Afnani, . D. A., Moses, . I. B., Farizqi, . M. T. I. & Kaben, . S. E. (2024) Detection of the methicillin-resistant Staphylococcus aureus (MRSA) gene encoding mecA in nasal swabs of cats in Surabaya, Indonesia. Open Veterinary Journal, 14 (11), 3026-3036. doi:10.5455/OVJ.2024.v14.i11.31


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly