E-ISSN 2218-6050 | ISSN 2226-4485
 
2026, Vol: 16, Issue: 1
16 / 1Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles
Required files to be uploaded

OVJ Manuscript Submissio Form
Title Page
mainarticle_template

Research Article


Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate

Christina Safira Whinie Lestari, Sabar Pambudi, Gissi Novientri, Fithriani Fithriani, Novaria Sari Dewi Panjaitan, Sunarno Sunarno, Monica Dwi Hartanti, Suprihatin Suprihatin.


Abstract
Background:
Dengue infection has increased significantly in subtropical and tropical regions in recent decades. As of 2020, 15 countries, including Indonesia, reported increasing cases, with mortality rates of approximately 0.5%–3.5%. Since 2014, the Dengue Vaccine Research Consortium under the Ministry of Health in the Republic of Indonesia has advanced a sharia-compliant dengue vaccine prototype in the form of the premembrane/envelope (prM/E) dengue virus (DENV)-1,-2,-3,-4 recombinant proteins.

Aim:
This study aims to develop and optimize an in-house indirect enzyme-linked immunosorbent assay (ELISA) for detecting IgG against prM/E DENV-3, serving as a preliminary antibody titer assessment for candidate dengue vaccines and potential diagnostic kits.

Methods:
Serum samples from mice immunized with 200 ng purified prM/E DENV-3 or 105 Plaque-forming units (PFU)/ml live DENV-3 or Phosphate-buffered saline (PBS) (negative control) were used. ELISA parameters, including antigen concentration (commercial DENV3), serum dilution, and conjugate antibody dilution, were systematically optimized. Optimal conditions were determined to be 100 ng antigen, 1:200 serum dilution, and 1:5,000 conjugate-Ab dilution.

Results:
Seropositive samples yielded absorbance values exceeding the established cutoff. The statistical analysis showed significant differences between groups (p < 0.001).

Conclusion:
The optimized in-house ELISA reliably detects IgG responses to recombinant prM/E DENV3, supporting its utility in early-stage evaluation of dengue vaccine candidates and in the development of diagnostic tools. This study is a successful stage supporting the development of prM/E DENV-1,-2,-3,-4 as a candidate dengue vaccine.

Key words: In-house ELISA; prM/E DENV-3; Dengue vaccine; Method optimization.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Christina Safira Whinie Lestari
Articles by Sabar Pambudi
Articles by Gissi Novientri
Articles by Fithriani Fithriani
Articles by Novaria Sari Dewi Panjaitan
Articles by Sunarno Sunarno
Articles by Monica Dwi Hartanti
Articles by Suprihatin Suprihatin
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Lestari CSW, Pambudi S, Novientri G, Fithriani F, Panjaitan NSD, Sunarno S, Hartanti MD, Suprihatin S. Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. Open Vet. J.. 2026; 16(1): 699-710. doi:10.5455/OVJ.2026.v16.i1.65

Web Style

Lestari CSW, Pambudi S, Novientri G, Fithriani F, Panjaitan NSD, Sunarno S, Hartanti MD, Suprihatin S. Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. https://www.openveterinaryjournal.com/?mno=267210 [Access: February 04, 2026]. doi:10.5455/OVJ.2026.v16.i1.65

AMA (American Medical Association) Style

Lestari CSW, Pambudi S, Novientri G, Fithriani F, Panjaitan NSD, Sunarno S, Hartanti MD, Suprihatin S. Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. Open Vet. J.. 2026; 16(1): 699-710. doi:10.5455/OVJ.2026.v16.i1.65


Vancouver/ICMJE Style

Lestari CSW, Pambudi S, Novientri G, Fithriani F, Panjaitan NSD, Sunarno S, Hartanti MD, Suprihatin S. Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. Open Vet. J.. (2026), [cited February 04, 2026]; 16(1): 699-710. doi:10.5455/OVJ.2026.v16.i1.65


Harvard Style

Lestari, C. S. W., Pambudi, . S., Novientri, . G., Fithriani, . F., Panjaitan, . N. S. D., Sunarno, . S., Hartanti, . M. D. & Suprihatin, . S. (2026) Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. Open Vet. J., 16 (1), 699-710. doi:10.5455/OVJ.2026.v16.i1.65


Turabian Style

Lestari, Christina Safira Whinie, Sabar Pambudi, Gissi Novientri, Fithriani Fithriani, Novaria Sari Dewi Panjaitan, Sunarno Sunarno, Monica Dwi Hartanti, and Suprihatin Suprihatin. 2026. Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. Open Veterinary Journal, 16 (1), 699-710. doi:10.5455/OVJ.2026.v16.i1.65


Chicago Style

Lestari, Christina Safira Whinie, Sabar Pambudi, Gissi Novientri, Fithriani Fithriani, Novaria Sari Dewi Panjaitan, Sunarno Sunarno, Monica Dwi Hartanti, and Suprihatin Suprihatin. "Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate." Open Veterinary Journal 16 (2026), 699-710. doi:10.5455/OVJ.2026.v16.i1.65


MLA (The Modern Language Association) Style

Lestari, Christina Safira Whinie, Sabar Pambudi, Gissi Novientri, Fithriani Fithriani, Novaria Sari Dewi Panjaitan, Sunarno Sunarno, Monica Dwi Hartanti, and Suprihatin Suprihatin. "Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate." Open Veterinary Journal 16.1 (2026), 699-710. Print. doi:10.5455/OVJ.2026.v16.i1.65


APA (American Psychological Association) Style

Lestari, C. S. W., Pambudi, . S., Novientri, . G., Fithriani, . F., Panjaitan, . N. S. D., Sunarno, . S., Hartanti, . M. D. & Suprihatin, . S. (2026) Optimization of in-house indirect ELISA for the detection of mice IgG anti-dengue prM/E DENV-3 as a vaccine candidate. Open Veterinary Journal, 16 (1), 699-710. doi:10.5455/OVJ.2026.v16.i1.65


Author LoginReviewer LoginAbout PublisherOpen Access PolicyEditorial Review PolicyEditorial & Peer Review ProcessPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyProtection of Research Participants (Statement On Human And Animal Rights)Corrections, Retractions & Expressions of ConcernDigital Archiving & Preservation PoliciesTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Editor-in-Chief

Open Veterinary Journal

Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldaghayes.com 

Emails: ibrahim.eldaghayes@vetmed.edu.ly

info@eldaghayes.com