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Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms

Raoudha Abbassi, Sabrine Rebhi, Sabah Dhibi, Ghada Ben Salah, Amal Ahmed Ismail, Eiman Ibrahim, Najla Hfaiedh, Assaad Sila, Samira Jebahi.


Abstract
Background:
Diabetes mellitus is associated with chronic hyperglycemia that induces oxidative stress and inflammatory responses, leading to progressive hepatic and renal dysfunction. Natural products rich in bioactive compounds, such as date seeds (Phoenix dactylifera), have gained increasing attention for their potential antioxidant, anti-inflammatory, and antidiabetic properties.

Aim:
This study investigated the effects of Kentichi date seed extracts on hepatic and renal function in a rat model of alloxan-induced diabetes.

Methods:
A total of 28 rats were randomly assigned to 4 experimental groups, with 7 individuals per group. Group I rats did not receive treatment. Group II rats were injected with alloxan (120 mg/kg body weight) for 2 consecutive days to induce diabetes. Group III rats were treated with alloxan (120 mg/kg body weight) and acarbose (5 mg/kg body weight) for 2 days. Group IV rats received alloxan (120 mg/kg body weight) and date seed extract (300 mg/kg body weight) for 2 days. Multiple biochemical markers, such as the enzymatic activity of α-amylase and α-glucosidase, the concentrations of C-reactive protein, tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6), as well as glycated hemoglobin (HbA1c), were measured. Oxidative stress parameters were also evaluated in hepatic and renal tissues, along with histopathological examinations.

Results:
The administration of date seed extract led to marked reductions in oral glucose tolerance test values, α-amylase and α-glucosidase activities, HbA1c, pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6), inducible nitric oxide synthase, and glutathione levels. The data suggest that the extract exerted significant antioxidant and anti-inflammatory effects, mitigating hepatic and renal damage and mass loss associated with alloxan-induced diabetes.

Conclusion:
The findings highlight the potential of date seed extract as a dietary supplement for individuals with compromised liver or kidney function.

Key words: Alloxan-induced diabetes; Anti-inflammatory; Date seed extract; Hepatorenal toxicity; Oxidative stress.


 
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How to Cite this Article
Pubmed Style

Abbassi R, Rebhi S, Dhibi S, Salah GB, Ismail AA, Ibrahim E, Hfaiedh N, Sila A, Jebahi S. Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. Open Vet. J.. 2026; 16(9): 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38

Web Style

Abbassi R, Rebhi S, Dhibi S, Salah GB, Ismail AA, Ibrahim E, Hfaiedh N, Sila A, Jebahi S. Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. https://www.openveterinaryjournal.com/?mno=316286 [Access: September 16, 2026]. doi:10.5455/OVJ.2026.v16.i9.38

AMA (American Medical Association) Style

Abbassi R, Rebhi S, Dhibi S, Salah GB, Ismail AA, Ibrahim E, Hfaiedh N, Sila A, Jebahi S. Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. Open Vet. J.. 2026; 16(9): 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38


Vancouver/ICMJE Style

Abbassi R, Rebhi S, Dhibi S, Salah GB, Ismail AA, Ibrahim E, Hfaiedh N, Sila A, Jebahi S. Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. Open Vet. J.. (2026), [cited September 16, 2026]; 16(9): 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38


Harvard Style

Abbassi, R., Rebhi, . S., Dhibi, . S., Salah, . G. B., Ismail, . A. A., Ibrahim, . E., Hfaiedh, . N., Sila, . A. & Jebahi, . S. (2026) Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. Open Vet. J., 16 (9), 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38


Turabian Style

Abbassi, Raoudha, Sabrine Rebhi, Sabah Dhibi, Ghada Ben Salah, Amal Ahmed Ismail, Eiman Ibrahim, Najla Hfaiedh, Assaad Sila, and Samira Jebahi. 2026. Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. Open Veterinary Journal, 16 (9), 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38


Chicago Style

Abbassi, Raoudha, Sabrine Rebhi, Sabah Dhibi, Ghada Ben Salah, Amal Ahmed Ismail, Eiman Ibrahim, Najla Hfaiedh, Assaad Sila, and Samira Jebahi. "Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms." Open Veterinary Journal 16 (2026), 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38


MLA (The Modern Language Association) Style

Abbassi, Raoudha, Sabrine Rebhi, Sabah Dhibi, Ghada Ben Salah, Amal Ahmed Ismail, Eiman Ibrahim, Najla Hfaiedh, Assaad Sila, and Samira Jebahi. "Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms." Open Veterinary Journal 16.9 (2026), 6322-6337. Print. doi:10.5455/OVJ.2026.v16.i9.38


APA (American Psychological Association) Style

Abbassi, R., Rebhi, . S., Dhibi, . S., Salah, . G. B., Ismail, . A. A., Ibrahim, . E., Hfaiedh, . N., Sila, . A. & Jebahi, . S. (2026) Date seed extract attenuates alloxan-induced hepatorenal toxicity in diabetic rats: Antioxidant and anti-inflammatory mechanisms. Open Veterinary Journal, 16 (9), 6322-6337. doi:10.5455/OVJ.2026.v16.i9.38


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