Abstract
Background:
A “Hepato Forte” is essentially a built-in substance in plants that reconditions the functional aspects of the liver to act in manners like hepatoprotective drugs, aiding in liver repair and acting as an effect enhancer.

Aim:
The current investigation examines the protective benefits of Hepato Forte on (lipid profiles, DNA damage, and glycemic index) in healthy hypercholesterolemic and phosphatidylcholine-deficient male rats.

Methods:
Thirty-two adult male albino rats were included, and there were 8 rats in each group. The treatments were administered orally daily to the rats. The control group, labeled C, received distilled water, while the second group was given Hepato Forte alone at a dose of (500 mg/kg/day) via gavage needle. A third group received cholesterol at a dose of (10 g/day) via gavage needle, and the fourth group received both Hepato Forte at 500 mg/kg/day and high cholesterol at a dose of 10 g/day.

Results:
Routine administration of Hepato Forte led to a significant decline (p > 0.05) in total cholesterol and triglycerides among both healthy and hypercholesterolemic male rats. Additionally, shows the effect of cholesterol and Hepato Forte on the concentrations and the body’s resistance to insulin in the T1 group, which decreased blood glucose and insulin resistance compared to the increased insulin levels in the same T1 group. In the T3 group of Agarose Gel Electrophoresis, it was observed that in animals the combination of cholesterol and Hepato Forte enhanced DNA integrity by reducing fragmentation and enhancing condensation and concentration through agarose gel electrophoresis.

Conclusion:
The findings of this research investigation show that Hepato Forte supplements and reducing DNA fragmentation play a critical protective role for the cardiovascular system and liver in adult male albino rats with hypercholesterolemia, effectively mitigating the adverse effects of high cholesterol on both the heart and liver.

Key words: Hepato Forte, Cholesterol, Insulin resistance, DNA, Hypercholesterolemia