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Research Article


Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia

Dewi Masyithah Darlan, Evita Mayasari, Sunna Vyatra Hutagalung, Ahadi Kurniawan, Ledy Afrida Sinaga, Alemina Pinem, Bernike Ambarita.


Abstract
Background:
Indonesia is a tropical country with heavy rainfall, mostly in low-lying areas. Floods are one of the most common natural disasters in Indonesia, with climate change causing continuous flooding in some regions. The spread of human pathogens as a severe consequence of flooding, such as Leptospira, which may cause Weil’s disease, is a concern for public health.

Aim:
In this cross-sectional study, we compared DNA from the rat kidney and serum samples to identify pathogenic Leptospira using polymerase chain reaction (PCR) amplification to promote a less invasive method of collecting samples from the rat vectors.

Methods:
Fifty-nine rodents inhabiting highly populated, flood-prone suburban regions were captured inside and outside the houses. Following DNA extractions, we analyzed the quantity and quality of DNA concentration from the kidney and serum specimens using a nanophotometer. The lipL32 gene was amplified to detect the pathogenic Leptospira.

Results:
The mean value of kidney DNA was 151.67 ng/µl with an average A260/A280 value of 1.836, whereas the mean value of serum DNA was 22.08 ng/µl with an average A260/A280 value of 1.233. Twenty (33.9%) kidney DNA and 10 (16.9%) serum DNA samples showed the target DNA (lipl32). The multiple sequence alignment (MSA) analysis revealed the lipL32 sequences homology to L. interrogans ser. Copenhageni.

Conclusion:
Rat kidneys exhibited higher DNA amount and purity than the serum. Moreover, PCR detection of lipl32 revealed higher positive results in kidney DNA than serum DNA samples, with high similarity to L. interrogans lipl32 sequences. Therefore, the kidney remains a better DNA source than serum for the molecular analysis of Leptospira in rats.

Key words: Kidney, Leptospira interrogans, lipL32, Rat, Serum


 
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How to Cite this Article
Pubmed Style

Darlan DM, Mayasari E, Hutagalung SV, Kurniawan A, Sinaga LA, Pinem A, Ambarita B. Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. Open Vet J. 2025; 15(1): 437-445. doi:10.5455/OVJ.2025.v15.i1.39

Web Style

Darlan DM, Mayasari E, Hutagalung SV, Kurniawan A, Sinaga LA, Pinem A, Ambarita B. Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. https://www.openveterinaryjournal.com/?mno=225182 [Access: March 12, 2025]. doi:10.5455/OVJ.2025.v15.i1.39

AMA (American Medical Association) Style

Darlan DM, Mayasari E, Hutagalung SV, Kurniawan A, Sinaga LA, Pinem A, Ambarita B. Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. Open Vet J. 2025; 15(1): 437-445. doi:10.5455/OVJ.2025.v15.i1.39


Vancouver/ICMJE Style

Darlan DM, Mayasari E, Hutagalung SV, Kurniawan A, Sinaga LA, Pinem A, Ambarita B. Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. Open Vet J. (2025), [cited March 12, 2025]; 15(1): 437-445. doi:10.5455/OVJ.2025.v15.i1.39


Harvard Style

Darlan, D. M., Mayasari, . E., Hutagalung, . S. V., Kurniawan, . A., Sinaga, . L. A., Pinem, . A. & Ambarita, . B. (2025) Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. Open Vet J, 15 (1), 437-445. doi:10.5455/OVJ.2025.v15.i1.39


Turabian Style

Darlan, Dewi Masyithah, Evita Mayasari, Sunna Vyatra Hutagalung, Ahadi Kurniawan, Ledy Afrida Sinaga, Alemina Pinem, and Bernike Ambarita. 2025. Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. Open Veterinary Journal, 15 (1), 437-445. doi:10.5455/OVJ.2025.v15.i1.39


Chicago Style

Darlan, Dewi Masyithah, Evita Mayasari, Sunna Vyatra Hutagalung, Ahadi Kurniawan, Ledy Afrida Sinaga, Alemina Pinem, and Bernike Ambarita. "Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia." Open Veterinary Journal 15 (2025), 437-445. doi:10.5455/OVJ.2025.v15.i1.39


MLA (The Modern Language Association) Style

Darlan, Dewi Masyithah, Evita Mayasari, Sunna Vyatra Hutagalung, Ahadi Kurniawan, Ledy Afrida Sinaga, Alemina Pinem, and Bernike Ambarita. "Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia." Open Veterinary Journal 15.1 (2025), 437-445. Print. doi:10.5455/OVJ.2025.v15.i1.39


APA (American Psychological Association) Style

Darlan, D. M., Mayasari, . E., Hutagalung, . S. V., Kurniawan, . A., Sinaga, . L. A., Pinem, . A. & Ambarita, . B. (2025) Molecular and serological detection of Leptospira interrogans among wild rats in flood-prone residential areas of Indonesia. Open Veterinary Journal, 15 (1), 437-445. doi:10.5455/OVJ.2025.v15.i1.39


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