E-ISSN 2218-6050 | ISSN 2226-4485
 
2025, Vol: 15, Issue: 2
15 / 2Current Issue ArchiveAims and ScopeAbstracting & IndexingMost Accessed ArticlesMost Downloaded ArticlesMost Cited Articles

Research Article


Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats

Barakat M. Alrashdi, Mahmoud Ashry, Mousa O. Germoush, Maged Fouda, Ibrahim Abdel-farid, Diaa Massoud, Fayez Shaldoum, Ahmed E. Abdel Moneim, Ali G. Gadel-rab, Mohamed Mahrous, Mohamed H.a. Gadelmawla, Hussam Askar.


Abstract
Background:
Exposure to carbon tetrachloride (CCl4) induces acute and chronic kidney damage alongside oxidative stress in rats.

Aim:
This study examines Nigella sativa ethanolic extract's (NEE) potential barriers against CCl4-induced nephrotoxicity.

Methods:
Wistar albino male rats weighing between 150 and 200g were acclimatized and randomly divided into four groups, each comprising 10 animals. The control group consisted of healthy rats; the second group received oral administration of 200 mg/kg NEE for six weeks; the third group received intraperitoneal injections of CCl4 at a dose of 0.5 mg/kg twice weekly for six weeks; and the fourth group received both oral NEE and CCl4.

Results:
Results indicate that NEE significantly mitigated renal degeneration induced by CCl4, evidenced by notable reductions in creatinine, urea, urea nitrogen, uric acid, potassium, IL-1β, IL-4, IL-6, IL-10, TNF-α, renal NO, MDA, and DNA fragmentation, coupled with substantial increases in kidney SOD, GPx, GSH, and CAT levels. Additionally, CD4, Albumin, Sodium, Calcium, Immunohistochemistry, and Histopathological analyses revealed marked regenerative effects.

Conclusion:
In conclusion, NEE exhibits anti-nephrotoxic, anti-inflammatory, and antioxidant properties, with a likely mediation by its antioxidant constituents. The radical scavenging activity, particularly the high phenolic content of its active component, suggests NEE's potential efficacy as a nephroprotective supplement.

Key words: Nephrotoxicity, CCL4, Nigella sativa, Anti-inflammatory, Antioxidant, Rat


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Barakat M. Alrashdi
Articles by Mahmoud Ashry
Articles by Mousa O. Germoush
Articles by Maged Fouda
Articles by Ibrahim Abdel-Farid
Articles by Diaa Massoud
Articles by Fayez Shaldoum
Articles by Ahmed E. Abdel Moneim
Articles by Ali G. Gadel-Rab
Articles by Mohamed Mahrous
Articles by Mohamed H.A. Gadelmawla
Articles by Hussam Askar
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Alrashdi BM, Ashry M, Germoush MO, Fouda M, Abdel-farid I, Massoud D, Shaldoum F, Moneim AEA, Gadel-rab AG, Mahrous M, Gadelmawla MH, Askar H. Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. Open Vet J. 2025; 15(1): 402-415. doi:10.5455/OVJ.2025.v15.i1.36

Web Style

Alrashdi BM, Ashry M, Germoush MO, Fouda M, Abdel-farid I, Massoud D, Shaldoum F, Moneim AEA, Gadel-rab AG, Mahrous M, Gadelmawla MH, Askar H. Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. https://www.openveterinaryjournal.com/?mno=226481 [Access: March 12, 2025]. doi:10.5455/OVJ.2025.v15.i1.36

AMA (American Medical Association) Style

Alrashdi BM, Ashry M, Germoush MO, Fouda M, Abdel-farid I, Massoud D, Shaldoum F, Moneim AEA, Gadel-rab AG, Mahrous M, Gadelmawla MH, Askar H. Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. Open Vet J. 2025; 15(1): 402-415. doi:10.5455/OVJ.2025.v15.i1.36


Vancouver/ICMJE Style

Alrashdi BM, Ashry M, Germoush MO, Fouda M, Abdel-farid I, Massoud D, Shaldoum F, Moneim AEA, Gadel-rab AG, Mahrous M, Gadelmawla MH, Askar H. Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. Open Vet J. (2025), [cited March 12, 2025]; 15(1): 402-415. doi:10.5455/OVJ.2025.v15.i1.36


Harvard Style

Alrashdi, B. M., Ashry, . M., Germoush, . M. O., Fouda, . M., Abdel-farid, . I., Massoud, . D., Shaldoum, . F., Moneim, . A. E. A., Gadel-rab, . A. G., Mahrous, . M., Gadelmawla, . M. H. & Askar, . H. (2025) Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. Open Vet J, 15 (1), 402-415. doi:10.5455/OVJ.2025.v15.i1.36


Turabian Style

Alrashdi, Barakat M., Mahmoud Ashry, Mousa O. Germoush, Maged Fouda, Ibrahim Abdel-farid, Diaa Massoud, Fayez Shaldoum, Ahmed E. Abdel Moneim, Ali G. Gadel-rab, Mohamed Mahrous, Mohamed H.a. Gadelmawla, and Hussam Askar. 2025. Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. Open Veterinary Journal, 15 (1), 402-415. doi:10.5455/OVJ.2025.v15.i1.36


Chicago Style

Alrashdi, Barakat M., Mahmoud Ashry, Mousa O. Germoush, Maged Fouda, Ibrahim Abdel-farid, Diaa Massoud, Fayez Shaldoum, Ahmed E. Abdel Moneim, Ali G. Gadel-rab, Mohamed Mahrous, Mohamed H.a. Gadelmawla, and Hussam Askar. "Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats." Open Veterinary Journal 15 (2025), 402-415. doi:10.5455/OVJ.2025.v15.i1.36


MLA (The Modern Language Association) Style

Alrashdi, Barakat M., Mahmoud Ashry, Mousa O. Germoush, Maged Fouda, Ibrahim Abdel-farid, Diaa Massoud, Fayez Shaldoum, Ahmed E. Abdel Moneim, Ali G. Gadel-rab, Mohamed Mahrous, Mohamed H.a. Gadelmawla, and Hussam Askar. "Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats." Open Veterinary Journal 15.1 (2025), 402-415. Print. doi:10.5455/OVJ.2025.v15.i1.36


APA (American Psychological Association) Style

Alrashdi, B. M., Ashry, . M., Germoush, . M. O., Fouda, . M., Abdel-farid, . I., Massoud, . D., Shaldoum, . F., Moneim, . A. E. A., Gadel-rab, . A. G., Mahrous, . M., Gadelmawla, . M. H. & Askar, . H. (2025) Anti-nephrotoxic, antioxidant and anti-inflammatory efficiency of Nigella sativa ethanolic extract against CCl4-induced nephrotoxicity in rats. Open Veterinary Journal, 15 (1), 402-415. doi:10.5455/OVJ.2025.v15.i1.36


Author LoginReviewer LoginAbout PublisherPublication Ethics and Publication Malpractice StatementConflict of Interest PolicyPlagiarism PolicyTerms of UseLicense InformationCopyright Information

About Open Veterinary Journal

Open Veterinary Journal provides a fast track coupled with high quality peer review process for original research articles, review articles, short communi ... Read more.



For best results, please use Internet Explorer or Google Chrome.

Contact Information

All correspondence should be addressed to:

Prof. Ibrahim Eldaghayes: Owner, Founder and Editor-in-Chief

Open Veterinary Journal


Faculty of Veterinary Medicine, University of Tripoli

P.O. Box 13662, Tripoli, Libya

and


Eldaghayes Publisher

www.eldagpublisher.com 

Email: ibrahim.eldaghayes@vetmed.edu.ly