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2025, Vol: 15, Issue: 2
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Research Article


Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract

Wurlina Wurlina, Imam Mustofa, Dewa Ketut Meles, Aswin Rafif Khairullah, Adeyinka Oye Akintunde, Kadek Rachmawati, Niluh Suwasanti, Dewa Made Sucipta Putra, Sri Mulyati, Suzanita Utama, Ulul Khoiriyah, Baich R. Tyarraushananda Defvyanto, Sila Faredy Heriana, Katty Hendriana Priscilia Riwu, Riza Zainuddin Ahmad, Audrey Gracelia Riwu.


Abstract
Background:
Lead intoxication triggers testicular toxicity via oxidative stress.

Aim:
This study aimed to explore the antioxidant potential of Moringa oleifera leaf extract (MOLE) in enhancing the semen quality of rats exposed to lead acetate.

Methods:
Twenty-five healthy rats were randomly and equally divided into five groups. Group C served as the negative control, whereas group C+ was exposed to lead acetate at 50 mg/kg body weight (BW)/day without MOLE. The T1, T2, and T3 groups were exposed to lead acetate at 50 mg/kg BW and concurrently received MOLE at doses of 200, 316, and 500 mg/kg BW/day, respectively, for 20 days. On the 21st day, all rats were euthanized for blood collection and testicle harvesting.

Results:
The result showed that exposure to lead acetate at 50 mg/kg BW/day in group C+ led to significant decreases (p < 0.05) in superoxide dismutase (SOD) levels, plasma membrane integrity (PMI), Leydig and Sertoli cell counts, spermatozoa numbers, sperm motility, and live spermatozoa, as well as significant increases (p < 0.05) in malondialdehyde (MDA) levels and apoptotic and necrotic sperm, compared with control group C–. The administration of MOLE to rats exposed to lead acetate resulted in improvement in all of these variables. However, SOD and testosterone levels, as well as spermatozoa numbers, viability, apoptosis, and necrosis, did not recover in group T3 (p < 0.05) compared with control group C–.

Conclusion:
MOLE effectively restores sperm quality in lead acetate–induced rats.

Key words: Leydig cells, malondialdehyde, membrane integrity, reproductive health, Sertoli cells


 
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How to Cite this Article
Pubmed Style

Wurlina W, Mustofa I, Meles DK, Khairullah AR, Akintunde AO, Rachmawati K, Suwasanti N, Putra DMS, Mulyati S, Utama S, Khoiriyah U, Defvyanto BRT, Heriana SF, Riwu KHP, Ahmad RZ, Riwu AG. Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. Open Vet J. 2025; 15(1): 416-427. doi:10.5455/OVJ.2025.v15.i1.37

Web Style

Wurlina W, Mustofa I, Meles DK, Khairullah AR, Akintunde AO, Rachmawati K, Suwasanti N, Putra DMS, Mulyati S, Utama S, Khoiriyah U, Defvyanto BRT, Heriana SF, Riwu KHP, Ahmad RZ, Riwu AG. Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. https://www.openveterinaryjournal.com/?mno=226188 [Access: March 12, 2025]. doi:10.5455/OVJ.2025.v15.i1.37

AMA (American Medical Association) Style

Wurlina W, Mustofa I, Meles DK, Khairullah AR, Akintunde AO, Rachmawati K, Suwasanti N, Putra DMS, Mulyati S, Utama S, Khoiriyah U, Defvyanto BRT, Heriana SF, Riwu KHP, Ahmad RZ, Riwu AG. Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. Open Vet J. 2025; 15(1): 416-427. doi:10.5455/OVJ.2025.v15.i1.37


Vancouver/ICMJE Style

Wurlina W, Mustofa I, Meles DK, Khairullah AR, Akintunde AO, Rachmawati K, Suwasanti N, Putra DMS, Mulyati S, Utama S, Khoiriyah U, Defvyanto BRT, Heriana SF, Riwu KHP, Ahmad RZ, Riwu AG. Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. Open Vet J. (2025), [cited March 12, 2025]; 15(1): 416-427. doi:10.5455/OVJ.2025.v15.i1.37


Harvard Style

Wurlina, W., Mustofa, . I., Meles, . D. K., Khairullah, . A. R., Akintunde, . A. O., Rachmawati, . K., Suwasanti, . N., Putra, . D. M. S., Mulyati, . S., Utama, . S., Khoiriyah, . U., Defvyanto, . B. R. T., Heriana, . S. F., Riwu, . K. H. P., Ahmad, . R. Z. & Riwu, . A. G. (2025) Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. Open Vet J, 15 (1), 416-427. doi:10.5455/OVJ.2025.v15.i1.37


Turabian Style

Wurlina, Wurlina, Imam Mustofa, Dewa Ketut Meles, Aswin Rafif Khairullah, Adeyinka Oye Akintunde, Kadek Rachmawati, Niluh Suwasanti, Dewa Made Sucipta Putra, Sri Mulyati, Suzanita Utama, Ulul Khoiriyah, Baich R. Tyarraushananda Defvyanto, Sila Faredy Heriana, Katty Hendriana Priscilia Riwu, Riza Zainuddin Ahmad, and Audrey Gracelia Riwu. 2025. Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. Open Veterinary Journal, 15 (1), 416-427. doi:10.5455/OVJ.2025.v15.i1.37


Chicago Style

Wurlina, Wurlina, Imam Mustofa, Dewa Ketut Meles, Aswin Rafif Khairullah, Adeyinka Oye Akintunde, Kadek Rachmawati, Niluh Suwasanti, Dewa Made Sucipta Putra, Sri Mulyati, Suzanita Utama, Ulul Khoiriyah, Baich R. Tyarraushananda Defvyanto, Sila Faredy Heriana, Katty Hendriana Priscilia Riwu, Riza Zainuddin Ahmad, and Audrey Gracelia Riwu. "Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract." Open Veterinary Journal 15 (2025), 416-427. doi:10.5455/OVJ.2025.v15.i1.37


MLA (The Modern Language Association) Style

Wurlina, Wurlina, Imam Mustofa, Dewa Ketut Meles, Aswin Rafif Khairullah, Adeyinka Oye Akintunde, Kadek Rachmawati, Niluh Suwasanti, Dewa Made Sucipta Putra, Sri Mulyati, Suzanita Utama, Ulul Khoiriyah, Baich R. Tyarraushananda Defvyanto, Sila Faredy Heriana, Katty Hendriana Priscilia Riwu, Riza Zainuddin Ahmad, and Audrey Gracelia Riwu. "Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract." Open Veterinary Journal 15.1 (2025), 416-427. Print. doi:10.5455/OVJ.2025.v15.i1.37


APA (American Psychological Association) Style

Wurlina, W., Mustofa, . I., Meles, . D. K., Khairullah, . A. R., Akintunde, . A. O., Rachmawati, . K., Suwasanti, . N., Putra, . D. M. S., Mulyati, . S., Utama, . S., Khoiriyah, . U., Defvyanto, . B. R. T., Heriana, . S. F., Riwu, . K. H. P., Ahmad, . R. Z. & Riwu, . A. G. (2025) Restoration of sperm quality in lead acetate-induced rats via treatment with Moringa oleifera leaf extract. Open Veterinary Journal, 15 (1), 416-427. doi:10.5455/OVJ.2025.v15.i1.37


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